Epigenetic changes can bring about new traits without altering the sequence of genes. This may allow plants to respond quicker to changes in their environment. Plant biologists at the University of Zurich have now demonstrated that epigenetic variation is also subject to selection and can be inherited. This could expand the possibilities for crop breeding.
Selection experiments with Arabidopsis show that epigenetic variation is also subject to selection and can be inherited. (Image: UZH)
The sequence of genes passed on to daughter cells or offspring isn’t the only factor that determines the traits of cells and organisms. Chemical changes in the genetic material that do not alter the underlying DNA sequence also play a role in controlling which genes are active or inactive. Methylation is one such epigenetic mark, which involves the addition of small chemical groups to specific bases in the DNA. The role of the inheritance of epigenetic variation in humans and mammals is controversial; however, there are several examples of epigenetic inheritance in plants.
Adaptability thanks to epigenetics
Plant biologists at the University of Zurich have now demonstrated that naturally occurring epigenetic variation in mouse-ear cress ( Arabidopsis thaliana
) is subject to selection. The team of Ueli Grossniklaus at the Department of Plant and Microbial Biology also showed that newly selected traits – which are important for seed dispersal – are passed on for at least two to three generations even without selection. “Epigenetic variation thus contributes to the ability of plants to quickly adapt to changes in the environment without sequence changes in the genome,” explains Grossniklaus.
Selection of plants with effective seed dispersal
In their experiment, the plant biologists simulated a rapidly changing environment. They selected Arabidopsis
populations over five generations according to how far they dispersed their seeds. Only seeds that spread to locations a certain distance from the mother plant were used for the subsequent generation. The researchers then took the seeds of three independent populations featuring effective seed dispersal and grew them together with seeds of the original, non-selected population – but this time in an environment without selection pressure. The plant populations were examined in depth after a further two generations.
Analysis of genetic activity, genome, and epigenome
“We were able to show that in the selected plants, two traits that are important for seed dispersal were different compared to the original population. The plants flowered later and had a higher number of branches,” says Grossniklaus. These changes could not be traced back to mutations in the genome of the plants. However, the researchers found significant differences in the epigenome: The state of methylation was altered at about 50,000 bases in the DNA. Differences were also found in the activity of genes that controlled flowering, for example.
New opportunities for crop breeding
Even under normal environmental conditions without selection, the new traits were maintained for at least two to three generations. “Like genetic variation, epigenetic variation is subject to selection and contributes to the diversity of plant traits. Since the genetic basis of crops is often very limited, epigenetics could be used to expand the material for plant breeding,” emphasizes Grossniklaus. Climate change is likely to alter the environmental conditions in many of the world’s regions within a short period of time. Plant species that can quickly adapt to changes are thus becoming increasingly important.
Marc W. Schmid, Christian Heichinger, Diana Coman Schmid, Daniela Guthörl, Valeria Gagliardini, Rémy Bruggmann, Sirisha Aluri, Catharine Aquino, Bernhard Schmid, Lindsay A. Turnbull, and Ueli Grossniklaus. Contribution of epigenetic variation to adaptation in Arabidopsis. Nature Communications. October 25, 2018. DOI: 10.1038/s41467-018-06932-5