Cell cytoskeleton as target for new active agents

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Tobias Mühlethaler in the PSI crystallisation facility, selecting suitable cryst

Tobias Mühlethaler in the PSI crystallisation facility, selecting suitable crystals for the measurements (Photo: Paul Scherrer Institute/Mahir Dzambegovic)

Through a unique combination of computer simulations and laboratory experiments, researchers at the Paul Scherrer Institute PSI have discovered new binding sites for active agents - against cancer, for example - on a vital protein of the cell cytoskeleton. Eleven of the sites hadn’t been known before. The study is published in the journal Angewandte Chemie International Edition.

The protein tubulin is an essential building block of the so-called cell cytoskeleton. In cells, tubulin molecules arrange themselves into tube-like structures, the microtubule filaments. These give cells their shape, aid in transporting proteins and larger cellular components, and play a crucial role in cell division.

Thus tubulin performs diverse functions in the cell and in doing so interacts with numerous other substances. "Tubulin can bind an astonishing number of different proteins and small molecules, several hundred for sure," says Tobias Mühlethaler, a doctoral candidate in the PSI Laboratory of Biomolecular Research and first author of the study. The functions of the protein are guided by means of such bonds. Also, many drugs dock on tubulin and take effect, for example, by preventing cell division in tumours.

"In this project, we addressed the fundamental question of how many binding sites in total exist on this vital protein," Mühlethaler explains. "If we discover new ones, these could possibly be used therapeutically."

From the virtual to the laboratory

In computer simulations conducted in collaboration with the Italian Institute of Technology in Genoa, the researchers combed through the structure of the protein: They identified places where other molecules could dock particularly well to tubulin. These are the so-called binding pockets.

Subsequently, in an actual laboratory experiment, the researchers sought to verify such sites. For this, they used a method called fragment screening: Starting with hundreds of crystals of tubulin, the researchers added individual solutions containing fragments of molecules that are typical precursors for promising active agents. Within an hour, the tubulin crystals were able to soak up as much of the fragment solution as they could hold. Finally the crystals were fished out of the liquid and exposed to synchrotron X-ray radiation. On the basis of the resulting diffraction pattern, the researchers are able to infer the structure of the crystal. Thus it could be determined if and where the molecule fragments have bound to the protein.

"Both methods, computer simulations and fragment screening, have their respective strengths and weaknesses," says Michel Steinmetz, head of the Laboratory of Biomolecular Research. "By combining them, we ensure that no binding site on the protein escapes our search."

Eleven new ones

Overall, the researchers found 27 binding sites on tubulin where molecules or other proteins can dock. "Eleven of them had never been described before," says Mühlethaler. In addition, the researchers identified 56 fragments that bind to tubulin and might be suitable for developing new active agents.

As the researchers stress, their approach is also transferable to other proteins. "Here we have developed a method for early discovery of so-called lead molecules and, with that, new starting points for the development of active agents," says Michel Steinmetz. It should be possible to apply this method successfully to all proteins for which high quality crystals can be obtained.

"The search for potential new lead molecules is a focus of the Swiss Light Source SLS," Steinmetz adds. "This will gain increasing significance after the upgrade to SLS 2.0, planned for the coming years, has taken place."

Text: Paul Scherrer Institute/Brigitte Osterath