Nanopore Detection of Single Molecule RNAP-DNA Transcription Complex

Nanopore Detection of Single Molecule RNAP-DNA Transcription Complex. In the past decade, a number of single-molecule methods have been developed with the aim of investigating single protein and nucleic acid interactions. For the first time the group of Aleksandra Radenovic (Laboratory of Nanoscale Biology) use solid-state nanopore sensing to detect a single E. coli RNAP-DNA transcription complex and single E. coli RNAP enzyme. On the basis of their specific conductance translocation signature, they can discriminate and identify between those two types of molecular translocations and translocations of bare DNA. This opens up a new perspectives for investigating transcription processes at the single-molecule level. C. Raillon et al. Nano Lett., 2 (3), pp 1157-1164 DOI: 10.1021/nl3002827 (2012)